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452045,452046,452047,452048,452049,452050 ECM 2001+ Electrofusion & Electroporation System BTX

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Powerful multi-purpose system providing fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines.



Brand: BTX
Part#: 452045,452046,452047,452048,452049,452050

Product Name Price Qty
452045(ECM 2001+ Cell Fusion System, Complete)
$31,928.00
45-2046 (ECM 2001+ Electroporation System Complete, for Cuvettes)
$28,404.00
45-2047(ECM 2001+ Embryo Manipulation System)
$29,597.00
45-2048(ECM 2001+ Hybridoma Production System, with ECM 2001+ 2 ml Coaxial Optimization Chamber)
$29,201.00
45-2049(ECM 2001+ Generator Only)
$28,251.00
45-2050(ECM 2001+ Electroporation System Complete, for Cuvettes with Monitoring)
$38,420.00

Details

The ECM 2001+ Electrofusion and Electroporation System provides fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines.

ECM 2001 GENERATOR

The ECM 2001+ is a multifunctional electrofusion and square wave electroporation generator. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.

The ECM 2001+ is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.

WAVEFORMS

AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.

FEATURES & BENEFITS

  • AC waveform of 0.2 – 2.0 MHz
  • Square wave electroporation capabilities
  • A wide range of voltages from 5 V to 3000 V
  • Fine voltage discrimination
  • Advanced programming capability to combine multiple AC steps pre- and post-DC fusion step
  • Capable of operating at low impedance loads
  • Large touchscreen interface


APPLICATIONS

ELECTROFUSION

Fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications are facilitated by the combination of AC and DC wave pulses. Fusion is achieved by the generation of an AC current wave form that generates a benign dielectrophoretic alignment of cells. With a 30 ms switchover time from AC to DC, efficient fusion takes place. After fusion, the AC is reapplied maintaining the cell compression for the rounding off process resulting in a higher number of hybrids.

ELECTROPORATION

Electroporation is a standard method used to transfect mammalian cell lines to express recombinant human proteins which are used for therapeutic purposes. Gene delivery by this method is typically used in transient transfections to study protein expression or to temporarily knockout or “silence” these genes using siRNA. This is used to study gene targeting and function.

Square waveform electroporation is an efficient non-viral method for transfecting genes and other molecules into mammalian cell lines. This technology is commonly used to study gene targeting, function and to understand protein regulation.

Alternatively, adding additional selection steps to isolate stably transfected cells allows for ;integration of a gene into the genome of the cell for long term expression of protein. The use of the ECM 2001+ offers the control needed to adjust electrical settings for optimization of parameters. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.


PRODUCT CONFIGURATIONS BY APPLICATION

Complete Cell Fusion and Electroporation System

For use in a wide range of cell fusion applications and mammalian cell electroporation, ECM 2001+ Cell Fusion System complete (45-2045) is our most versatile instrument configuration. The ECM 2001+ Cell Fusion system complete comes with a selection of fusion chambers (Microslides, Meander Fusion Chamber, Flat Electrode) as well as safety stand and cuvettes for electroporation.

Hybridoma Production

For use in large volume cell fusion applications such as hybridoma production, the ECM 2001+ generator, 2 ml optimization coaxial chamber, and 9 ml production coaxial chamber are used. This system (98-6169) does not require proprietary fusion medium, but can be used with the Cytofusion Medium Cfor convenience. Coaxial chambers (47-0030, 47-0020) are also available for purchase separately.

Embryo Manipulation

For use in small volume cell fusion applications such as embryo manipulation, we recommend the ECM 2001+ Embryo Manipulation System (45-2047). In this system the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.

Mammalian Cell Transfections

Utilizing the ECM 2001+ System Complete, for Cuvettes (45-2046) for standard mammalian cell transfections is simple. The AC feature is turned off and the DC mode is set as a square wave electroporation device. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 10 pulses per experiment, make this an extremely versatile system for any lab.

Adherent Cell Transfections

Electroporate adherent cells directly into the dish used for cell growth. The ECM 2001+ coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for 100 mm petri dishes.

Square Wave Pulse, DC
Voltage Range
   LV Mode 5 to 500 V in 1 V steps
    HV Mode 505 to 3000 V in 1 V steps
Voltage Accuracy
   LV Mode 5%
   HV Mode 5%
Pulse Length Range
   LV Mode 10 μs to 999 μs in 1 μs steps
1 ms to 999 ms in 1 ms steps
   HV Mode 10 μs to 600 μs in 1 μs steps
Multiple Pulsing
   LV Mode 1 to 10 pulses per sample
   HV Mode 1 to 10 pulses per sample
Pulse Interval 0.1 s to 10 s
Alignment (Pre-Pulse), AC
Frequency 0.2 to 2 MHz in 0.1 MHz steps
Voltage 5 to 75 V in 5 V steps
 Duration 0 to 99 s in 1 s steps
Wave Shape Sine wave
Post-Fusion, AC 
Frequency  0.2 to 2 MHz in 0.1 MHz steps
Voltage 5 to 75 V in 5 V steps
Duration 0 to 99 s in 1 s steps
Wave Shape Sine wave
Sample Load Ranges
AC
   All Voltages Load must be ≥60 Ω>
DC
   LV Mode Pulse Length <100 ms,
Load must be >8 to 9 Ω,
Pulse Length >100 ms,
Load must be >100 Ω
   HV Mode> Pulse Length ≤600 ms
Load must be ≥40 Ω
Other Specifications
 Charging Time  LV = 7 s, HV = 7 s
DC Monitoring Protocol Name, Steps Executed,
 Time/Date, Error Conditions, Pulse
 Voltage, Pulse Current,
 Pulse Width, Pulse #, Droop %,
 Sample Voltage, Sample Current and
 Sample Resistance
Display 7-inch full color displayy
Controls  Touch Screen
Programmability Stores over 1000 protocols
 DC Arc Control Yes
Pre-Pulse Sample Load Check Ves (20 V DC)
Computer (Optional) PC running Microsoft Windows XP, 7, 8.1 or 10
Communications USB
Data File Format Comma Separated Values (CSV)
Remote Operation  Yes, using Foot Pedal control
Accessory Connector Type Coaxial
Power Ratings 70 W idle and 900 W pulsing
 Input Voltage Ratings  100 to 240 VAC, 50 / 60 Hz
Dimensions (H x W x D)  13 x 12 x 13 in
Weight 22 lb
Atmospheric Specifications
   Operating Temperature 4°C to 40°C (40°F to 104°F)
   Storage Temperature -10°C to 70°C (14°F to 158°F)
   Operating Humidity 20% to 80% RH, non-condensing
   Storage Humidity 20% to 80% RH, non-condensing
Mode of Operation Continuous
Classification Class I
Pollution IP2X
Installation Category II
 Regulatory Certifications  CE, ETL (UL, CSA), FCC, WEEE, EU RoHS & CB Scheme

Additional Information

Brand BTX

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