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450010,450011,450012,450013,450018 Legacy ECM 2001 Electro Cell Fusion & Electroporation System BTX

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he Legacy ECM 2001 multi-purpose electro cell manipulation generator produces a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion.



Brand: BTX
Part#: 450010,450011,450012,450013,450018

Product Name Price Qty
45-0018INT(Legacy ECM 2001 Hybridoma Production System)
$20,232.00
450012INT(Legacy ECM 2001 Embryo Manipulation System)
$21,154.00
45-0013INT(Legacy ECM 2001 Electroporation System Complete, for Cuvettes with Monitoring)
$30,419.00
45-0018(Legacy ECM 2001 Hybridoma Production System (N. America)
$20,739.00
45-0013(Legacy ECM 2001 Electroporation System Complete, for Cuvettes with Monitoring (N. America)
$30,419.00
45-0012(Legacy ECM 2001 Embryo Manipulation System (N. America)
$21,154.00
45-0011(Legacy ECM 2001 Electroporation System Complete, for Cuvettes (N. America)
$19,902.00
45-0010(Legacy ECM 2001 Cell Fusion System, Complete (N. America)
$23,601.00

Details

The Legacy ECM 2001 multi-purpose electro cell manipulation generator produces a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion.

LEGACY ECM 2001 GENERATOR The LEGACY ECM 2001 is a multipurpose electro cell manipulation pulse generator capable of performing both electrofusion for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined with standard cuvettes, high throughput plates, specialty electrodes and other available accessories.

The Legacy ECM 2001 is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

FEATURES

  • Wide range of voltages from 5 to 3000 V
  • Finer voltage discrimination
  • Pulse durations from 10 µsec to 10 sec/li>
  • Arc quenching
  • Digital display of actual voltage and pulse length delivered

FUSION
The Legacy ECM 2001 is capable of producing a proprietary AC waveform for benign dielectrophoretic alignment of cells, and a square DC waveform for cell fusion. Electrofusion is carried out by applying both the AC and DC pulse waveforms. Microsecond switch-over time from AC to DC allows for highly efficient fusion. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse for the rounding off process; resulting in a higher number of hybrids. The 1 MHz AC pulse aligns cells together in real time, saving time (seconds vs. hours) in comparison to PEG methods. Blastomeres and oocytes can also be aligned to the correct position for better fusion accuracy during nuclear transfer and embryo manipulation.

ELECTROPORATION
The Legacy ECM 2001 EP System is designed for transfection of eukaryotic cells in suspension usingelectroporation cuvettes

Utilizing the Legacy ECM 2001 for standard mammalian cell transfections is simple; the AC feature is turned off and the DC mode is set as a square wave electroporation device. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 9 pulses per experiment, make this an extremely versatile system for any lab. 

ADHERENT CELL TRANSFECTION
Electroporate adherent cells directly into the dish used for cell growth. The Legacy ECM 2001 coupled with thePetri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for 100 mm petri dishes.

HYBRIDOMA PRODUCTION
For use in hybridoma production applications, the Legacy ECM 2001 generator, 3.2 mm gap or 10 mm gap Microslides, and connection cables are required. This system does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience.
Electrofusion is achieved by the generation of a proprietary 1 MHz AC waveform for benign dielectrophoretic cell alignment, microsecond switchover from AC to DC creates efficient fusion results. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse resulting in a higher number of hybrids.

AC PARAMETERS (ALIGNMENT)
Proprietary nonsinusoidal wave shape
Frequency 1 MHz
Voltage 0 - 75 Vrms
Duration 0 - 99 sec
Post fusion AC 1/10 of pre-fusion amplitude
Post fusion ramp 1 - 9 sec
Pause between AC/DC 50 µsec
DC PULSE PARAMETERS (FUSION) (ELECTROPORATION)
Intended Use Indoor use only
High Voltage Mode
Voltage 10 - 3,000 V
Pulse Length 1 - 99 µsec/1 µsec resolution
Low Voltage Mode
Voltage 10 - 500 V
Pulse Length 0.01 - 0.99 msec/ 0.01 msec res ,1 - 99 msec/ 1 msec res
Number of Pulses 1 - 9 pulses
Pulse Interval 1 sec fixed
Number of Post Cycle Repeats 0 - 9 cycles
Capacitance LV Mode: 3,900 µF / HV Mode: 108 µF
Resistance LV Mode: 50 ohms / HV mode: 50 ohms
Safety Generator is short-circuit proof
Remote Operation Automatic or manually controlled switch
MECHANICAL CHARACTERISTICS
Footprint 17" x 15.5" x 11" ( W x D x H)
Weight 47 lb (21.3 kg)
Controls Thumbwheel potentiometer and push button control

Additional Information

Brand BTX

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